yes tune first cause after you lean it out youll pick up more rpm and at higher rpm the shaft will spin faster causing it to engage 2nd gear try this first
and how would i lockitite it? do you mean just to take the whole screw out and then locktite it and then find the perfect setting?
as for loctite its however you want to do it you couold find the right spot that you want and when you remove screw count turns so you know were to stop after you put the loctite in cause if you put loctite in them try to adjust you might only get one chance before loctite dries