Agree with bluelevel. UAT is marginal for even 120, due to the soft sides of the bottle, which start to collapse under vacuum, reducing the working area on the mesh trap. . You want a larger capacity and rigid sides. Take a look at GBR CATs. They come with larger fittings to accommodate 5/16 Tygon. You want to run 5/16 almost all the way to the pump if possible.

Apart from this, it is risky to safety wire the big tygon to a barb deigned for 1/4 Tygon. No matter how tight you get the wire, it may still pull in air at the fitting. Not worth the risk.

Also, be careful using 3/16 fittings inside the tank. You may need to go one tube size up to 7/32 (solder over the 3/16 to ensure a secure fit. If you are using 5/16 Viton inside the tank, you will need to go 1/4 to ensure snug fit. That's even with safety wire.