I always used 12 or 14 on my 40 size trainers, on something like an Avistar with a 46, 14/16 would be what I would use. having to many might hurt your wing more in a crash... not having enough could give you a lawn dart

I also removed my rubber bands with a Knife at the end of the day... I've seen some guy's that would wash them, dip them in talc/corn starch and so on, so they could reuse them... If 3 bucks worth of NEW rubber bands is to much to spend a month your in the wrong hobby.
John