Ye, ye. I once maidened a GWS e-starter, my first aileron plane. On pre-flight, I just checked if all the surfaces were moving, didn't pay attention to the orientation. Take-off was great 'til I tried to bank into a turn. Plane responded opposite my stick input. Thought it was the wind so I gave it more stick. Shortest flight I ever had. Ailerons were set-up reversed!
Even better is to have someone really experienced to maiden it for you. He'll know what to expect on a first flight and have the plane trimmed out.