What is a nitro line? Do you mean a glow fuel line? Most glow fuel lines are silicon base and can NOT be used with gasoline. As to filler valves, depends on what they are made of. some are compatible with both alcohol and gasoline while some are not. Those made of metal can be used with just about all types of fuel, some plastics can not. I would suspect the fine print on the containers should tell you whether or not they can be used with specific types of fuel.