I looked at my transmitter (DX5e) that came with the sim and all channels are set to normal except the ailerons. It is the only one that is reversed. It has been like this since new and have only had to calibrate one time (when installed).

Make sure everything is set to norm except the ailerons. Do this with the TX off and unhooked from the USB cord. Once you have done that; go back through the set up process and see if that fixes the problem.